EFFECTS OF HYPOTAURINE AND CYSTEINE IN WASHING SOLUTIONS ON COOLED AND FROZEN THAW GOAT SPERMATOZOA

  • A. A. Memon Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan
  • H. Wahid Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia
  • Y. Rosnina Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia
  • Y. M. Goh Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia
  • A. N. Tunio Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan
Keywords: Antioxidants, cryopreservation, cysteine, hypotaurine, solutions, washing

Abstract

This study was conducted to determine the effect of hypotaurine and cysteine (antioxidant) in washing solution (Tris citric acid fructose extender without egg yolk) on motility, membrane integrity, morphology, acrosome integrity and viabilty of cooled and frozen thaw boer goat spermatozoa. Significantly better results in terms of motility and morphology of cooled spermatozoa have been observed when antioxidants were added in Tris citric acid fructose extender washing solution. Furthermore, significant improvement was observed by supplementation of tris citric acid fructose extender in terms of motility and acrosome integrity compared to non-supplemented groups after freezing. In conclusion, antioxidants can be added in washing solution in order to reduce the oxidative stress in cooled and frozen boer goat spermatozoa.

Published
2014-06-30