EFFECTS OF HYPOTAURINE AND CYSTEINE IN WASHING SOLUTIONS ON COOLED AND FROZEN THAW GOAT SPERMATOZOA
Keywords:Antioxidants, cryopreservation, cysteine, hypotaurine, solutions, washing
This study was conducted to determine the effect of hypotaurine and cysteine (antioxidant) in washing solution (Tris citric acid fructose extender without egg yolk) on motility, membrane integrity, morphology, acrosome integrity and viabilty of cooled and frozen thaw boer goat spermatozoa. Significantly better results in terms of motility and morphology of cooled spermatozoa have been observed when antioxidants were added in Tris citric acid fructose extender washing solution. Furthermore, significant improvement was observed by supplementation of tris citric acid fructose extender in terms of motility and acrosome integrity compared to non-supplemented groups after freezing. In conclusion, antioxidants can be added in washing solution in order to reduce the oxidative stress in cooled and frozen boer goat spermatozoa.