DETERMINATION OF EARLY PREGNANCY THROUGH PLASMA PROGESTERONE CONCENTRATION IN KAMORI GOAT
Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan
Keywords:ELISA, goat, Kamori, pregnancy, progesterone
An early pregnancy was determined through plasma progesterone concentration in Kamori goat, under semi-intensive management condition. Thirty adult normal cycling Kamori goats were selected and divided in to three equal (N-10) treatment groups and synchronized with different estrus synchronization protocols. Goats in group A were given progesterone on day 0 and PGF2? with GnRH on day 10th , goats in group B were given PGF2? on day-0 and repeat dose of PGF2? with GnRH on day 10th, whereas goats in group C served as control. All the goats were observed for estrus induction. Goats showing estrus were allowed for breeding, natural breeding was practiced using an intact breeding bucks. The blood samples were collected after day 21st of post-breeding to measure progesterone concentration level with ELISA Reader (Beck-Man Coulter Inc.AD-430) using Progesterone kit (Accu-Bind®, Monobind Inc. USA). The mean (±SEM) progesterone concentration level was recorded as 2.42±1.37, 2.57±1.21, 2.71±1.06 in group A, B, and C, respectively in pregnant and 1.27±0.98 ng/ml in non-pregnant goats. No significant difference (P> 0.05) was found among the goat groups A, B and C. Increased progesterone concentration level (post-breeding) in goats was found to be one of the best indicators of pregnancy. This study suggests that the ELISA kit method could be used for early pregnancy determination in Kamori goats.